Composite
Part:BBa_K5311020:Design
Designed by: Carlos Perez de Arenaza Group: iGEM24_UPF-Barcelona (2024-09-27)
MG_010 + RNA thermometer ForU + CAP 10-3 Endolysin + His-tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 367
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 135
Illegal NgoMIV site found at 546 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 294
Design Notes
For this composite part we have codon optimized the CDS for the RNA Thermometer to be more compatible with C. acnes, we also introduced the standard prefix and sufixes in our sequence to ensure a proper alignment for the Gibson Assembly.
Source
This part was created by using the corresponding sequences on this registry: MG10:BBa_J23119Part:; RNA Thermometer: Part:BBa_K115002; Endolysin: Part:BBa_K5311006 and the His-tag corresponds to a sequence of 6 Hystidines