Composite

Part:BBa_K5311020:Design

Designed by: Carlos Perez de Arenaza   Group: iGEM24_UPF-Barcelona   (2024-09-27)


MG_010 + RNA thermometer ForU + CAP 10-3 Endolysin + His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 367
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 135
    Illegal NgoMIV site found at 546
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 294


Design Notes

For this composite part we have codon optimized the CDS for the RNA Thermometer to be more compatible with C. acnes, we also introduced the standard prefix and sufixes in our sequence to ensure a proper alignment for the Gibson Assembly.


Source

This part was created by using the corresponding sequences on this registry: MG10:BBa_J23119Part:; RNA Thermometer: Part:BBa_K115002; Endolysin: Part:BBa_K5311006 and the His-tag corresponds to a sequence of 6 Hystidines

References